imagej software program 7.0a (GraphPad Software Inc)
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Imagej Software Program 7.0a, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
Images
1) Product Images from "Gamma irradiation exposure for collapsed cell junctions and reduced angiogenesis of 3-D in vitro blood vessels"
Article Title: Gamma irradiation exposure for collapsed cell junctions and reduced angiogenesis of 3-D in vitro blood vessels
Journal: Scientific Reports
doi: 10.1038/s41598-021-97692-8
Figure Legend Snippet: Immunofluorescence images of F-actin and quantification of actin stress fibers in hMVECs after gamma irradiation. ( a ) The structure and alignment of the actin filaments in the hMVECs changed after the gamma irradiation exposure. ( b ) Representative immunofluorescence images of F-actin and quantification for the percentage of cells with different numbers of actin stress fibers after gamma irradiation. (scale bar: 50 µm, * p < 0.05; ** p < 0.01; *** p < 0.001, n = 3 (15 randomly chosen hMVECs = 1)). Statistical comparison were calculated with two-tailed t-tests using Prism (7.0a, GraphPad software, www.graphpad.com ). Images were analyzed using ImageJ (1.52q, NIH, www.imagej.nih.gov/ij ). All the images and graph were created with Adobe illustrator (CC 2019 23.0.1, www.adobe.com ).
Techniques Used: Immunofluorescence, Irradiation, Comparison, Two Tailed Test, Software
Figure Legend Snippet: Representative fluorescence images and calculated permeability values of endothelial barriers after irradiation. ( a ) The tight hMVECs’ monolayer showed a sharp decrease in the intensity ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\Delta {\varvec{C}}$$\end{document} Δ C ) at the interface between the hMVECs and the remodeled ECM. In addition, the intensity gradient \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\left( {\frac{{\partial {\varvec{C}}}}{{\partial {\varvec{x}}}}} \right)$$\end{document} ∂ C ∂ x in the ECM was measured in the normalized intensity profile with ImageJ (1.52q, NIH, www.imagej.nih.gov/ij ). These intensity values vary according to the barrier permeability. (b) The permeability values of the hMVECs’ monolayer after 0, 4, and 8 Gy of gamma irradiation treatment (scale bar: 100 and 200 µm; * p < 0.05; ** p < 0.01; *** p < 0.001, n = 4). Statistical comparison were calculated with two-tailed t-tests using Prism (7.0a, GraphPad software, www.graphpad.com ). Images and graphs were created with Adobe illustrator (CC 2019 23.0.1, www.adobe.com ).
Techniques Used: Fluorescence, Permeability, Irradiation, Comparison, Two Tailed Test, Software